For each measured sample, at least 5–6 cells were measured. Quantification of corrected total cell fluorescence (CTCF) of F-actin for (c) MCF-7 as received and MCF-7 cells grown on 3D bone-mimetic scaffolds (d) MM 231 as received and MM 231 cells grown on 3D bone-mimetic scaffolds. Scaffolds grown MM 231 neither formed an F-actin band between adjacent cells nor stress-fibers. Scaffolds grown MCF-7 formed an F-actin band (shown by white arrows) between adjacent cells (b) MM 231 as received and MM 231 cells grown on 3D bone-mimetic scaffolds. Representative immunofluorescence images showing distribution of F-actin in (a) MCF-7 as received and MCF-7 cells grown on 3D bone-mimetic scaffolds. Intersections with the horizontal dashed line at tan δ = 1 of the vertical lines occur at transition frequencies, ωtransition. Variation of loss tangent (tan δ) for (e) MCF-7 as received and 3D bone-mimetic scaffolds-derived MCF-7 cells (f) MM 231 as received and 3D bone-mimetic scaffolds-derived MM 231 cells. Variation of storage modulus (E′\documentclass) for (c) MCF-7 as received and 3D bone-mimetic scaffolds-derived MCF-7 cells (d) MM 231 as received and 3D bone-mimetic scaffolds-derived MM 231 cells. Taken together, our data demonstrate evolution of breast cancer cell mechanics at bone metastases. Our results showed that the significant reduction in elastic modulus along with increased liquid-like behavior of bone metastasized MCF-7 cells was induced by depolymerization and reorganization of F-actin to the adherens junctions, whereas bone metastasized MDA-MB-231 cells showed insignificant changes in elastic modulus and F-actin reorganization over time, compared to their respective as-received counterparts. Here, we report evaluation of mechanical properties of two breast cancer cells with different metastatic ability at the site of bone metastases, using quasi-static and dynamic nanoindentation methods. Reorganization of cytoskeleton facilitates the colonization of metastatic breast cancer at bone marrow space, leading to bone metastasis. In recent years, there has been increasing interest in investigating the mechanical properties of individual cells to delineate disease mechanisms.
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